Journal
NUCLEIC ACIDS RESEARCH
Volume 41, Issue 22, Pages 10438-10448Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkt832
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Funding
- Canadian Institutes for Health Research [MOP 79344]
- Saskatchewan Health Research Fund [2570]
- Natural Sciences and Engineering Research Council [RGPIN 326797-2011]
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Spirochetes of the genus Borrelia include the tick-transmitted causative agents of Lyme disease and relapsing fever. They possess unusual genomes composed mainly of linear replicons terminated by closed DNA hairpin telomeres. Hairpin telomeres present an uninterrupted DNA chain to the replication machinery overcoming the 'end-replication problem' for the linear replicons. Hairpin telomeres are formed from inverted repeat replicated telomere junctions by the telomere resolvase, ResT. ResT uses a reaction mechanism similar to that of the type IB topoisomerases and tyrosine recombinases. We report here that ResT also possesses single-strand annealing activity and a limited ability to promote DNA strand exchange reactions on partial duplex substrates. This combination of activities suggests ResT is a nexus between the seemingly distinct processes of telomere resolution and homologous recombination. Implications for hairpin telomere replication and linear plasmid recombination, including antigenic variation, are discussed.
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