Journal
NUCLEIC ACIDS RESEARCH
Volume 40, Issue 16, Pages 8033-8047Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gks506
Keywords
-
Categories
Funding
- Ministry of Education, Science, Sports and Culture of Japan
- New Energy and Industrial Technology Development Organization (NEDO)
- Japan Ministry of Education, Science, Sports and Culture
- Grants-in-Aid for Scientific Research [22227006, 23655153] Funding Source: KAKEN
Ask authors/readers for more resources
In human mitochondria, 10 mRNAs species are generated from a long polycistronic precursor that is transcribed from the heavy chain of mitochondrial DNA, in theory yielding equal copy numbers of mRNA molecules. However, the steady-state levels of these mRNAs differ substantially. Through absolute quantification of mRNAs in HeLa cells, we show that the copy numbers of all mitochondrial mRNA species range from 6000 to 51 000 molecules per cell, indicating that mitochondria actively regulate mRNA metabolism. In addition, the copy numbers of mitochondrial mRNAs correlated with their cellular half-life. Previously, mRNAs with longer half-lives were shown to be stabilized by the LRPPRC/SLIRP complex, which we find that cotranscriptionally binds to coding sequences of mRNAs. We observed that the LRPPRC/SLIRP complex suppressed 3' exonucleolytic mRNA degradation mediated by PNPase and SUV3. Moreover, LRPPRC promoted the polyadenylation of mRNAs mediated by mitochondrial poly(A) polymerase (MTPAP) in vitro. These findings provide a framework for understanding the molecular mechanism of mRNA metabolism in human mitochondria.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available