Journal
NUCLEIC ACIDS RESEARCH
Volume 41, Issue 4, Pages 2354-2369Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gks1347
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Funding
- Cambridge University Commonwealth Trust
- UK Medical Research Council
- BBSRC [BB/F012802/1] Funding Source: UKRI
- MRC [MC_U105663140, MC_U105697135, MC_U105674181, MC_UP_1202/14] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/F012802/1] Funding Source: researchfish
- Medical Research Council [MC_U105674181, MC_UP_1202/14, MC_U105697135, MC_U105663140] Funding Source: researchfish
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Alternative translation initiation (ATI) is a mechanism of producing multiple proteins from a single transcript, which in some cases regulates trafficking of proteins to different cellular compartments, including mitochondria. Application of a genome-wide computational screen predicts a cryptic mitochondrial targeting signal for 126 proteins in mouse and man that is revealed when an AUG codon located downstream from the canonical initiator methionine codon is used as a translation start site, which we term downstream ATI (dATI). Experimental evidence in support of dATI is provided by immunoblotting of endogenous truncated proteins enriched in mitochondrial cell fractions or of co-localization with mitochondria using immunocytochemistry. More detailed cellular localization studies establish mitochondrial targeting of a member of the cytosolic poly(A) binding protein family, PABPC5, and of the RNA/DNA helicase PIF1 alpha. The mitochondrial isoform of PABPC5 co-immunoprecipitates with the mitochondrial poly(A) polymerase, and is markedly reduced in abundance when mitochondrial DNA and RNA are depleted, suggesting it plays a role in RNA metabolism in the organelle. Like PABPC5 and PIF1 alpha, most of the candidates identified by the screen are not currently annotated as mitochondrial proteins, and so dATI expands the human mitochondrial proteome.
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