Journal
NUCLEIC ACIDS RESEARCH
Volume 40, Issue 14, Pages 6765-6773Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gks381
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Funding
- European Social Fund [VP1-3.1-SMM-07-K-01-105]
- Lithuanian State Science and Studies Foundation
- French Ministry of foreign and European affairs [SUT-157]
- Excellence Initiative of the German Federal and State Governments
- FP7-REGPOT-2009-1 program [245721]
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Biophysical and mechanistic investigation of RNA function requires site-specific incorporation of spectroscopic and chemical probes, which is difficult to achieve using current technologies. We have in vitro reconstituted a functional box C/D small ribonucleoprotein RNA 2'-O-methyltransferase (C/D RNP) from the thermophilic archaeon Pyrococcus abyssi and demonstrated its ability to transfer a prop-2-ynyl group from a synthetic cofactor analog to a series of preselected target sites in model tRNA and pre-mRNA molecules. Target selection of the RNP was programmed by changing a dodecanucleotide guide sequence in a 64-nt C/D guide RNA leading to efficient derivatization of three out of four new targets in each RNA substrate. We also show that the transferred terminal alkyne can be further appended with a fluorophore using a bioorthogonal azide-alkyne 1,3-cycloaddition (click) reaction. The described approach for the first time permits synthetically tunable sequence-specific labeling of RNA with single-nucleotide precision.
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