Journal
NUCLEIC ACIDS RESEARCH
Volume 40, Issue 15, Pages 7176-7189Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gks351
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Funding
- National Institutes of Health [GM70566]
- National Science Foundation [IIS1016929]
- PhRMA predoctoral fellowship in Informatics
- Department of Biochemistry, SUNY at Buffalo
- Div Of Information & Intelligent Systems
- Direct For Computer & Info Scie & Enginr [1016929] Funding Source: National Science Foundation
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We have used micrococcal nuclease (MNase) digestion followed by deep sequencing in order to obtain a higher resolution map than previously available of nucleosome positions in the fission yeast, Schizosaccharomyces pombe. Our data confirm an unusually short average nucleosome repeat length, similar to 152 bp, in fission yeast and that transcriptional start sites (TSSs) are associated with nucleosome-depleted regions (NDRs), ordered nucleosome arrays downstream and less regularly spaced upstream nucleosomes. In addition, we found enrichments for associated function in four of eight groups of genes clustered according to chromatin configurations near TSSs. At replication origins, our data revealed asymmetric localization of pre-replication complex (pre-RC) proteins within large NDRs-a feature that is conserved in fission and budding yeast and is therefore likely to be conserved in other eukaryotic organisms.
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