Journal
NUCLEIC ACIDS RESEARCH
Volume 40, Issue 6, Pages 2611-2622Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkr1102
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Funding
- National Cancer Institute at the University of Vermont (USA) [R01CA52040]
- Kempestiftelserna
- Swedish Research Council
- Swedish Cancer Society
- Smartafonden
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The biological role of human DNA polymerase theta (POLQ) is not yet clearly defined, but it has been proposed to participate in several cellular processes based on its translesion synthesis capabilities. POLQ is a low-fidelity polymerase capable of efficient bypass of blocking lesions such as abasic sites and thymine glycols as well as extension of mismatched primer termini. Here, we show that POLQ possesses a DNA polymerase activity that appears to be template independent and allows efficient extension of single-stranded DNA as well as duplex DNA with either protruding or multiply mismatched 3'-OH termini. We hypothesize that this DNA synthesis activity is related to the proposed role for POLQ in the repair or tolerance of double-strand breaks.
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