Journal
NUCLEIC ACIDS RESEARCH
Volume 39, Issue 3, Pages 1131-1141Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkq810
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Funding
- National Institutes of Health [AI-15706, AI-16892]
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We have generated a series of variable-strength, constitutive, bacterial promoters that act predictably in different sequence contexts, span two orders of magnitude in strength and contain convenient sites for cloning and the introduction of downstream open-reading frames. Importantly, their design insulates these promoters from the stimulatory or repressive effects of many 5'- or 3'-sequence elements. We show that different promoters from our library produce constant relative levels of two different proteins in multiple genetic contexts. This set of promoters should be a useful resource for the synthetic-biology community.
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