Journal
NUCLEIC ACIDS RESEARCH
Volume 38, Issue 15, Pages -Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkq556
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Funding
- National Natural Science Foundation [20725516, 90913014, 30871362, 10975179]
- National Basic Research Program of China [2007CB936000]
- Ministry of Health [2009ZX10004-301]
- Shanghai Municipal Commission for Science and Technology [0952nm04600]
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We herein report the design of a dumbbell-shaped DNA probe that integrates target-binding, amplification and signaling within one multifunctional design. The dumbbell probe can initiate rolling circle amplification (D-RCA) in the presence of specific microRNA (miRNA) targets. This D-RCA-based miRNA strategy allows quantification of miRNA with very low quantity of RNA samples. The femtomolar sensitivity of D-RCA compares favorably with other existing technologies. More significantly, the dynamic range of D-RCA is extremely large, covering eight orders of magnitude. We also demonstrate miRNA quantification with this highly sensitive and inexpensive D-RCA strategy in clinical samples.
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