Journal
NUCLEIC ACIDS RESEARCH
Volume 38, Issue 19, Pages -Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkq684
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Funding
- Deutsche Forschungsgemeinschaft [DFG SPP 1356, SFB 646, 684]
- Elite Network of Bavaria
- International Max Planck Research School for Molecular and Cellular Life Sciences (IMPRS-LS)
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The recent discovery of genomic 5-hydroxymethylcytosine (hmC) and mutations affecting the respective Tet hydroxylases in leukemia raises fundamental questions about this epigenetic modification. We present a sensitive method for fast quantification of genomic hmC based on specific transfer of radiolabeled glucose to hmC by a purified glucosyltransferase. We determined hmC levels in various adult tissues and differentiating embryonic stem cells and show a correlation with differential expression of tet genes.
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