4.8 Article

Targeted resequencing of candidate genes using selector probes

Journal

NUCLEIC ACIDS RESEARCH
Volume 39, Issue 2, Pages -

Publisher

OXFORD UNIV PRESS
DOI: 10.1093/nar/gkq1005

Keywords

-

Funding

  1. Knut and Alice Wallenberg
  2. Goran Gustafsson
  3. Erik K Fernstrom foundations
  4. Swedish Research Council
  5. VINNOVA
  6. EU [HEALTH-F4-2008-201418]

Ask authors/readers for more resources

Targeted genome enrichment is a powerful tool for making use of the massive throughput of novel DNA-sequencing instruments. We herein present a simple and scalable protocol for multiplex amplification of target regions based on the Selector technique. The updated version exhibits improved coverage and compatibility with next-generation-sequencing (NGS) library-construction procedures for shotgun sequencing with NGS platforms. To demonstrate the performance of the technique, all 501 exons from 28 genes frequently involved in cancer were enriched for and sequenced in specimens derived from cell lines and tumor biopsies. DNA from both fresh frozen and formalin-fixed paraffin-embedded biopsies were analyzed and 94% specificity and 98% coverage of the targeted region was achieved. Reproducibility between replicates was high (R-2 = 0, 98) and readily enabled detection of copy-number variations. The procedure can be carried out in < 24 h and does not require any dedicated instrumentation.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.8
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available