Journal
NUCLEIC ACIDS RESEARCH
Volume 37, Issue 11, Pages 3531-3544Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkp214
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Funding
- Ministry of Education, Culture, Sports, Science, and Technology (MEXT)
- Naito foundation
- Japan Society for the Promotion of Science (JSPS) [19790316, 19657002, 1770001]
- University of Tokyo
- Grants-in-Aid for Scientific Research [19790316, 19657002] Funding Source: KAKEN
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Cleavage of a DNA replication fork leads to fork restoration by recombination repair. In prokaryote cells carrying restrictionmodification systems, fork passage reduces genome methylation by the modification enzyme and exposes the chromosome to attack by the restriction enzyme. Various observations have suggested a relationship between the fork and Type I restriction enzymes, which cleave DNA at a distance from a recognition sequence. Here, we demonstrate that a Type I restriction enzyme preparation cleaves a model replication fork at its branch. The enzyme probably tracks along the DNA from an unmethylated recognition site on the daughter DNA and cuts the fork upon encountering the branch point. Our finding suggests that these restrictionmodification systems contribute to genome maintenance through cell death and indicates that DNA replication fork cleavage represents a critical point in genome maintenance to choose between the restoration pathway and the destruction pathway.
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