Journal
NUCLEIC ACIDS RESEARCH
Volume 37, Issue 5, Pages -Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkp043
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Funding
- UK BBSRC
- Wellcome Trust
- Biotechnology and Biological Sciences Research Council [BB/C501484/1] Funding Source: researchfish
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We describe a protocol, DNA sampling, for the rapid isolation of specific segments of DNA, together with bound proteins, from Escherichia coli K-12. The DNA to be sampled is generated as a discrete fragment within cells by the yeast I-SceI meganuclease, and is purified using FLAG-tagged LacI repressor and beads carrying anti-FLAG antibody. We illustrate the method by investigating the proteins bound to the colicin K gene regulatory region, either before or after induction of the colicin K gene promoter.
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