Journal
NUCLEIC ACIDS RESEARCH
Volume 36, Issue 20, Pages 6406-6417Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkn686
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Funding
- National Natural Science Foundation of China [30530180]
- National Protein Project of Ministry of Science and Technology [2006CB910 902]
- Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-YW-R63]
- Ministry of Science and Technology [2004CB720004]
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Rad9 is conserved from yeast to humans and plays roles in DNA repair (homologous recombination repair, and base-pair excision repair) and cell cycle checkpoint controls. It has not previously been reported whether Rad9 is involved in DNA mismatch repair (MMR). In this study, we have demonstrated that both human and mouse Rad9 interacts physically with the MMR protein MLH1. Disruption of the interaction by a single-point mutation in Rad9 leads to significantly reduced MMR activity. This disruption does not affect S/M checkpoint control and the first round of G(2)/M checkpoint control, nor does it alter cell sensitivity to UV light, gamma rays or hydroxyurea. Our data indicate that Rad9 is an important factor in MMR and carries out its MMR function specifically through interaction with MLH1.
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