Journal
NUCLEIC ACIDS RESEARCH
Volume 36, Issue 5, Pages 1497-1507Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkm1179
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Funding
- NIGMS NIH HHS [R01 GM058859, GM058859] Funding Source: Medline
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To ensure accurate and rapid protein synthesis, nearby and distantly located functional regions of the ribosome must dynamically communicate and coordinate with one another through a series of information exchange networks. The ribosome is 2/3 rRNA and information should pass mostly through this medium. Here, two viable mutants located in the peptidyltransferase center (PTC) of yeast ribosomes were created using a yeast genetic system that enables stable production of ribosomes containing only mutant rRNAs. The specific mutants were C2820U (Escherichia coli C2452) and 2922C (E. coli U2554). Biochemical and genetic analyses of these mutants suggest that they may trap the PTC in the open or aa-tRNA bound conformation, decreasing peptidyl-tRNA binding. We suggest that these structural changes are manifested at the biological level by affecting large ribosomal subunit biogenesis, ribosomal subunit joining during initiation, susceptibility/resistance to peptidyltransferase inhibitors, and the ability of ribosomes to properly decode termination codons. These studies also add to our understanding of how information is transmitted both locally and over long distances through allosteric networks of rRNArRNA and rRNAprotein interactions.
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