Journal
NUCLEIC ACIDS RESEARCH
Volume 36, Issue 20, Pages 6548-6557Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkn703
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Funding
- North Carolina State University RNA Biology Group
- Mitochondrial Disease Foundation [05-20]
- NSF [MCB0548602]
- Dharmacon RNA Technologies, Inc.
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Human mitochondrial methionine transfer RNA (hmtRNA(CAU)(MET)) has a unique post-transcriptional modification, 5-formylcytidine, at the wobble position-34 (f(5)C(34)). The role of this modification in hmtRNA(CAU)(MET) for the decoding of AUA, as well as AUG, in both the peptidyl- and aminoacyl-sites of the ribosome in either chain initiation or chain elongation is still unknown. We report the first synthesis and analyses of the tRNA's anticodon stem and loop domain containing the 5-formylcytidine modification. The modification contributes to the tRNA's anticodon domain structure, thermodynamic properties and its ability to bind codons AUA and AUG in translational initiation and elongation.
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