Mutation induction of Pleurotus ferulae by low-energy N+ ion implantation and characters of the selected mutant

In order to obtain Pleurotus ferulae with high temperature tolerance, mycelium mono-cells of wild type strain ACK was treated by nitrogen ion (5-30 keV, 1.5x10(15) similar to 1.5 x 10(16) cm(-2)) implantation, and mutant CGMCC1762 was selected through auxotrophy screening method, which was Lys, VB6 auxotrophy stress with high temperature. We found that during riper period the surface layer mycelium of the mutant was not aging neither grew tegument even above 30 degrees C. The mycelium endurable temperature of the mutant was increased 7 degrees C compared with that of the wild type strain. The fruiting bodies growth temperature of the mutant was 16 similar to 20 degrees C in daytime and was 6 similar to 12 degrees C at night. The highest growth temperature of fruiting bodies of the mutant was increased by PC than that of original strain. Through three generation investigation, we found that the mutant CGMCC1762 was stable with high temperature tolerance.