Journal
NUCLEAR MEDICINE COMMUNICATIONS
Volume 32, Issue 2, Pages 147-154Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/MNM.0b013e328341b268
Keywords
bioluminescence; carcino-embryonic antigen; colon carcinoma; liver metastasis; pretargeting; radioimmunotherapy
Ask authors/readers for more resources
Background Radioimmunotherapy is emerging as a new tool for adjuvant therapy of colorectal cancer. The liver remains the main site for metastases, carrying a high mortality rate. Many animal models are available but none associates easy, reliable implantation and in-vivo follow-up for experimental therapeutic studies. The aims of this study were to develop a reliable hepatic metastatic colonic cancer model in mice using the intraportal route for injection, with follow-up by bioluminescence (BLI) and to evaluate the impact of tumor location on tumor antigen direct targeting using radiolabeled anti-CEA (carcinoembryonic antigen) antibodies. Methods Ls-174T Luc+ is a colon carcinoma cell line strongly expressing CEA, transfected with the luciferase gene for BU. Isolated or aggregated cells (1 x 10(6)) were injected through the portal vein. The tumor burden was investigated using BLI to assess hepatic implantation and growth kinetics. The biodistribution of the I-125 anti-CEA antibody fragment (F6) was studied in this model and was compared with subcutaneous implantation. Results The tumor implantation rate was 100% using aggregated cells compared with 26.6% of isolated cells. Photons emitted by 1 x 10(6) cells were detected by BLI immediately after injection and allowed visual confirmation of hepatic distribution. The tumor growth was assessed over time to select homogeneous groups of animals. Radiolabeled anti-CEA antibody biodistributions showed a significantly higher uptake in hepatic than in subcutaneous tumors. Conclusion The association of hepatic tumor graft through the portal route and BLI provides a reliable animal model and permits sensitive in-vivo detection and follow-up of hepatic metastases. The hepatic model seems to more closely reproduce colon cancer metastases compared with subcutaneous metastasis. The hepatic model is of particular interest for studying radioimmunotherapy. Nucl Med Commun 32:147-154 (C) 2011 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins. Nuclear Medicine Communications 2011, 32:147-154
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available