Journal
NUCLEAR MEDICINE AND BIOLOGY
Volume 36, Issue 2, Pages 191-197Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.nucmedbio.2008.11.009
Keywords
Diffuse large B-cell lymphoma; Glucose transporter; Hexokinase; FDG-PET
Ask authors/readers for more resources
Introduction: Diffuse large B-cell lymphoma (DLBCL) has been reported to show higher uptake of 2-deoxy-2-F18-fluoro-D-glucose (FDG) by positron emission tomography than other B-cell non-Hodgkin's lymphomas (non-DLBCL). The authors addressed the mechanism of FDG uptake in DLBCL by immunostaining for glucose transporter Types 1 (Glut-1) and 3 (Glut-3) and hexokinase-II (HK-II) in excised lymphoma tissues. Methods: Sixteen B-cell non-Hodgkin's lymphoma patients (11 DLBCL and 5 non-DLBCL patients) were included in the study because the lymphoma tissues obtained by excision were eligible for immunostaining. The expressions of Glut-1. Glut-3 and HK-II were assessed regarding the percentages of positively stained lymphoma cells (%expression), the staining intensities (none=0, weak=1, moderate=2 and strong=3) and the staining patterns (membranous or cytoplasmic) and compared between DLBCL and non-DLBCL. Results: Glut-1 was not expressed at all in DLBCL or non-DLBCL, and their Glut-3 expressions were not significantly different (P >.05) with respect to %expression (mean +/- S.E.M., 73.6 +/- 7.3% vs. 72.0 +/- 3.7%), staining intensity (2.5 +/- 0.2 vs. 2.6 +/- 0.2) and staining pattern (membranous pattern dominant: 54.5% vs. 60.0%). However, DLBCL expressed more HK-II than non-DLBCL, i.e.,%expression (45.2 +/- 11.5% vs. 17.0 +/- 15.8%, P=.0275) and staining intensity (2.3 +/- 0.2 vs. 0.6 +/- 0.4, P=.0032). HK-II showed a cytoplasmic location in DLBCL and non-DLBCL. Conclusions: HK-II and Glut-3 contribute significantly to FDG uptake in DLBCL. DLBCL may have higher FDG uptake because it expresses more HK-II, whereas Glut-1 appears to play no role in FDG uptake in B-cell non-Hodgkin's lymphoma. (c) 2009 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available