Journal
NMR IN BIOMEDICINE
Volume 25, Issue 4, Pages 506-513Publisher
WILEY-BLACKWELL
DOI: 10.1002/nbm.1763
Keywords
glioma-initiating cells; xenografts in nude mice; NMR spectroscopy; metabolic profile
Funding
- European Commission [MRTN-CT-2006-035801]
- Centre d'Imagerie BioMedicale (CIBM) of the UNIL
- Centre d'Imagerie BioMedicale (CIBM) of the UNIGE
- Centre d'Imagerie BioMedicale (CIBM) of the HUG
- Centre d'Imagerie BioMedicale (CIBM) of the CHUV
- Centre d'Imagerie BioMedicale (CIBM) of the EPFL
- Leenaards Foundation
- Jeantet Foundation
- Anita et Werner Damm-Etienne Foundation
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In the last decade, evidence has emerged indicating that the growth of a vast majority of tumors including gliomas is sustained by a subpopulation of cancer cells with stem cell properties called cancer initiating cells. These cells are able to initiate and propagate tumors and constitute only a fraction of all tumor cells. In the present study, we showed that intracerebral injection of cultured glioma-initiating cells into nude mice produced fast growing tumors showing necrosis and gadolinium enhancement in MR images, whereas gliomas produced by injecting freshly purified glioma-initiating cells grew slowly and showed no necrosis and very little gadolinium enhancement. Using proton localized spectroscopy at 14.1 Tesla, decreasing trends of N-acetylaspartate, glutamate and glucose concentrations and an increasing trend of glycine concentration were observed near the injection site after injecting cultured glioma-initiating cells. In contrast to the spectra of tumors grown from fresh cells, those from cultured cells showed intense peaks of lipids, increased absolute concentrations of glycine and choline-containing compounds, and decreased concentrations of glutamine, taurine and total creatine, when compared with a contralateral non-tumor-bearing brain tissue. A decrease in concentrations of N-acetylaspartate and -aminobutyrate was found in both tumor phenotypes after solid tumor formation. Further investigation is needed to determine the cause of the dissimilarities between the tumors grown from cultured glioma-initiating cells and those from freshly purified glioma-initiating cells, both derived from human glioblastomas. Copyright (C) 2011 John Wiley & Sons, Ltd.
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