Journal
NMR IN BIOMEDICINE
Volume 24, Issue 5, Pages 506-513Publisher
WILEY
DOI: 10.1002/nbm.1616
Keywords
hyperpolarized three-dimensional C-13 MRSI; [1-C-13]pyruvate; hepatocellular carcinoma; alanine transaminase
Funding
- National Institute of health (NIH) of the United States [CA-09695, RR-09784, AA-018681, EB-009070, AA-005965]
- T. S. Kwok Foundation
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Hepatocellular carcinoma (HCC), the primary form of human adult liver malignancy, is a highly aggressive tumor with average survival rates that are currently less than 1 year following diagnosis. Most patients with HCC are diagnosed at an advanced stage, and no efficient marker exists for the prediction of prognosis and/or response(s) to therapy. We have reported previously a high level of [1-C-13] alanine in an orthotopic HCC using single-voxel hyperpolarized [1-C-13] pyruvate MRS. In the present study, we implemented a three-dimensional MRSI sequence to investigate this potential hallmark of cellular metabolism in rat livers bearing HCC (n = 7 buffalo rats). In addition, quantitative real-time polymerase chain reaction was used to determine the mRNA levels of lactate dehydrogenase A, nicotinamide adenine (phosphate) dinucleotide dehydrogenase quinone 1 and alanine transaminase. The enzyme levels were significantly higher in tumor than in normal liver tissues within each rat, and were associated with the in vivo MRSI signal of [1-C-13] alanine and [1-C-13] lactate after a bolus intravenous injection of [1-C-13] pyruvate. Histopathological analysis of these tumors confirmed the successful growth of HCC as a nodule in buffalo rat livers, revealing malignancy and hypervascular architecture. More importantly, the results demonstrated that the metabolic fate of [1-C-13] pyruvate conversion to [1-C-13] alanine significantly superseded that of [1-C-13] pyruvate conversion to [1-C-13] lactate, potentially serving as a marker of HCC tumors. Copyright (C) 2010 John Wiley & Sons, Ltd.
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