Journal
NITRIC OXIDE-BIOLOGY AND CHEMISTRY
Volume 26, Issue 1, Pages 74-80Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.niox.2011.12.003
Keywords
Mast cells; Nitric oxide; Peroxynitrite; Nitration; 15-Hydroxy prostaglandin dehydrogenase
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Mast cells (MC) play a pivotal role in allergic inflammation and nitric oxide (NO) is known to regulate MC function. One mechanism of NO mediated actions is the post-translational modification protein tyrosine nitration mediated by reactive nitrogen species. In this study we identified targets for nitration in the human mast cell line LAD2 after treatment with a nitric oxide donor and with peroxynitrite. Using two dimensional gel electrophoresis and western blot analyses with monoclonal and polyclonal antibodies we identified 15-hydroxy prostaglandin dehydrogenase (PGDH), a major prostaglandin catabolizing enzyme, as a target for nitration in LAD2. This is the first report on expression of this enzyme in MC and also the first report that PGDH is a target of protein tyrosine nitration. Since MC synthesize and metabolize many prostaglandins including prostaglandin E-2, the major substrate for PGDH, nitration of this prostaglandin catabolizing enzyme is likely functionally significant. (C) 2011 Elsevier Inc. All rights reserved.
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