Journal
NITRIC OXIDE-BIOLOGY AND CHEMISTRY
Volume 23, Issue 3, Pages 214-219Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.niox.2010.06.005
Keywords
Gold nanoparticles; Lipopolysaccharide (LPS); Signal transducer and activator of transcription 1 (STAT1); Nitric oxide (NO); Inducible nitric synthase (iNOS); Interferon-beta (IFN-beta)
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Funding
- Regional Innovation Center of the Ministry of Knowledge Economy, through Bio-Food & Drug Research Center at Konkuk University, Korea
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Macrophage-derived nitric oxide (NO) plays an important role in protection against microbial infection in immune responses. Overproduction of NO by inducible nitric synthase (iNOS) is known to be closely correlated with the pathology of a variety of diseases and inflammations. In this study, we investigated the inhibitory effect of polyethylene glycol coated gold nanoparticles (GNP) on NO production and its molecular mechanism in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. It was found that GNP inhibited LPS-induced NO production and iNOS expression in RAW264.7 cells. Furthermore, GNP suppressed LPS-induced activation of NF-kappa B through the inhibition of Akt activity. GNP also inhibited LPS-induced phosphorylation of signal transducer and activator of transcription 1 (STAT1) via down-regulation of interferon-beta (IFN-beta) expression. Our results suggest that GNP inhibits NO production and iNOS expression through blocking the activation of NF-kappa B and STAT1 in LPS-stimulated RAW264.7 cells. (C) 2010 Elsevier Inc. All rights reserved.
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