The role of sulfur dioxide as an endogenous gaseous vasoactive factor in synergy with nitric oxide

To explore the physiological role of endogenous gaseous sulfur dioxide (SO2) on vascular contractility and its underlying cellular and molecular mechanisms, vasodilation experiment of isolated rat thoracic aortic rings by gaseous SO2 was carried out and the signal transduction pathways involved in the vascular effects of SO2 were investigated. In the present study, SO2 gas and SO2 gas-bubbled solution (SO2 stock solution) were first used to relax vascular tissues. The results show: (1) Gaseous SO2 relaxed rat thoracic aortic rings in a dose-dependent manner (from 1 to 2000 PM). The vasorelaxant effect of SO2 at physiological relevant and low concentrations (<450 mu M) was endothelium-dependent, and at high concentrations (>500 mu M) was endothelium-independent. (2) The vasorelaxation by addition of SO2 stock solution (final concentrations <= 2 mM) was actually caused by SO2 molecules, not by sulfite or bisulfite, and the characteristic of vasorelaxation by SO2 was different from that of sulfite and bisulfite. (3) The vasorelaxant effect of Sot was not due to the altered neurotransmitter release from the autonomous or nonadrenergic and noncholinergic (NANC) nerve endings, also not due to superoxide and hydrogen peroxide produced in the vascular tissues, also disapproving the involvement of prostaglandin, PKC, P-adrenoceptor and cAMP pathways. (4) The vasorelaxant effect of SO2 at the physiological relevant and low concentrations was mediated by the cGMP pathway. (5) There was the synergistic effect on smooth muscle relaxation between much lower concentrations of SO2 (3 mu M) and NO (3 or 5 nM). These findings led to the conclusions: endogenous gaseous SO2 was a vasoactive factor, which might regulate vascular smooth muscle tone in synergy with NO. (C) 2008 Elsevier Inc. All rights reserved.