Journal
STRUCTURE
Volume 23, Issue 12, Pages 2267-2279Publisher
CELL PRESS
DOI: 10.1016/j.str.2015.10.010
Keywords
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Funding
- Knut and Alice Wallenberg Foundation
- Swedish Cancer Foundation
- Swedish Child Cancer Foundation
- Carl Trygger foundation
- LiU Cancer Research Network
- Swedish Research Council
- NCI [R01s CA129040, CA100855]
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Hierarchic phosphorylation and concomitant Pin1-mediated proline isomerization of the oncoprotein c-Myc controls its cellular stability and activity. However, the molecular basis for Pin1 recognition and catalysis of c-Myc and other multisite, disordered substrates in cell regulation and disease is unclear. By nuclear magnetic resonance, surface plasmon resonance, and molecular modeling, we show that Pin1 subdomains jointly pre-anchor unphosphorylated c-Myc(1-88) in the Pin1 interdomain cleft in a disordered, or fuzzy'', complex at the herein named Myc Box 0 (MB0) conserved region N-terminal to the highly conserved Myc Box I (MBI). Ser62 phosphorylation in MBI intensifies previously transient MBI-Pin1 interactions in c-Myc(1-88) binding, and increasingly engages Pin1(PPIase) and its catalytic region with maintained MB0 interactions. In cellular assays, MB0 mutated c-Myc shows decreased Pin1 interaction, increased protein half-life, but lowered rates of Myc-driven transcription and cell proliferation. We propose that dynamic Pin1 recognition of MB0 contributes to the regulation of c-Myc activity in cells.
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