Journal
STRUCTURE
Volume 23, Issue 6, Pages 1087-1096Publisher
CELL PRESS
DOI: 10.1016/j.str.2015.04.003
Keywords
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Funding
- Chinese Ministry of Science and Technology 973 program [2011CB910304, 2011CB911103]
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDB08020200]
- Chinese Academy of Sciences [KJZD-EW-L02]
- National Natural Science Foundation of China [31100535]
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Cerebral cavernous malformation 2 (CCM2) functions as an adaptor protein implicated in various biological processes. By interacting with the mitogen-activated protein kinase MEKK3, CCM2 either mediates the activation of MEKK3 signaling in response to osmotic stress or negatively regulates MEKK3 signaling, which is important for normal cardiovascular development. However, the molecular basis governing CCM2-MEKK3 interaction is largely unknown. Here we report the crystal structure of the CCM2 C-terminal part (CCM2ct) containing both the five-helix domain (CCM2ct(s)) and the following C-terminal tail. The end of the C-terminal tail forms an isolated helix, which interacts intramolecularly with CCM2ct(s). By biochemical studies we identified the N-terminal amphiphilic helix of MEKK3 (MEKK3-n(helix)) as the essential structural element for CCM2ct binding. We further determined the crystal structure of CCM2ct(s)-MEKK3-n(helix) complex, in which MEKK3-n(helix) binds to the same site of CCM2ct(s) for CCM2ct intramolecular interaction. These findings build a structural framework for understanding CCM2ct-MEKK3 molecular recognition.
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