Journal
NEW PHYTOLOGIST
Volume 201, Issue 4, Pages 1328-1342Publisher
WILEY
DOI: 10.1111/nph.12611
Keywords
Aphanomyces euteiches; F-box protein; Genome-wide association study; Medicago truncatula; oomycete; partial resistance; root
Categories
Funding
- CNRS
- Universite Paul Sabatier
- INRA
- French Agence Nationale de la Recherche [ANR-10-GENM-0007]
- US National Science Foundation [IOS-1237993]
- NGCR
- Agence Nationale de la Recherche (ANR) [ANR-10-GENM-0007] Funding Source: Agence Nationale de la Recherche (ANR)
- Direct For Biological Sciences
- Division Of Integrative Organismal Systems [1237993] Funding Source: National Science Foundation
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The use of quantitative disease resistance (QDR) is a promising strategy for promoting durable resistance to plant pathogens, but genes involved in QDR are largely unknown. To identify genetic components and accelerate improvement of QDR in legumes to the root pathogen Aphanomyces euteiches, we took advantage of both the recently generated massive genomic data for Medicago truncatula and natural variation of this model legume. A high-density (approximate to 5.1 million single nucleotide polymorphisms (SNPs)) genome-wide association study (GWAS) was performed with both in vitro and glasshouse phenotyping data collected for 179 lines. GWAS identified several candidate genes and pinpointed two independent major loci on the top of chromosome 3 that were detected in both phenotyping methods. Candidate SNPs in the most significant locus (sigma(2)(A) = 23%) were in the promoter and coding regions of an F-box protein coding gene. Subsequent qRT-PCR and bioinformatic analyses performed on 20 lines demonstrated that resistance is associated with mutations directly affecting the interaction domain of the F-box protein rather than gene expression. These results refine the position of previously identified QTL to specific candidate genes, suggest potential molecular mechanisms, and identify new loci explaining QDR against A. euteiches.
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