Journal
NEW PHYTOLOGIST
Volume 194, Issue 2, Pages 353-363Publisher
WILEY-BLACKWELL
DOI: 10.1111/j.1469-8137.2012.04062.x
Keywords
Arabidopsis; BY-2 cells; DNA double-strand breaks (DSBs); DNA repair; E2F factor; H2AX; repair foci
Categories
Funding
- Action Concertee (Biologie Moleculaire Cellulaire et Structurale)
- Ministere de l'Education Nationale et de la Recherche
- Centre National de la Recherche Scientifique
- University of Strasbourg
- Region Alsace
- Association de la Recherche sur le Cancer
- Ligue Nationale contre le Cancer
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Cellular responses to DNA double-strand breaks (DSBs) are linked in mammals and yeasts to the phosphorylated histones H2AX (gamma H2AX) repair foci which are multiproteic nuclear complexes responsible for DSB sensing and signalling. However, neither the components of these foci nor their role are yet known in plants. In this paper, we describe the effects of gamma H2AX deficiency in Arabidopsis thaliana plants challenged with DSBs in terms of genotoxic sensitivity and E2F-mediated transcriptional responses. We further establish the existence, restrictive to the G1/S transition, of specific DSB-induced foci containing tobacco E2F transcription factors, in both A. thaliana roots and BY-2 tobacco cells. These E2F foci partially colocalize with gamma H2AX foci while their formation is ataxia telangiectasia mutated (ATM)-dependent, requires the E2F transactivation domain with its retinoblastoma-binding site and is optimal in the presence of functional H2AXs. Overall, our results unveil a new interplay between plant H2AX and E2F transcriptional activators during the DSB response.
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