Journal
NEW PHYTOLOGIST
Volume 194, Issue 2, Pages 430-439Publisher
WILEY
DOI: 10.1111/j.1469-8137.2012.04052.x
Keywords
3-deoxy-D-arabino-heptulosonate 7-phosphate synthase; Arabidopsis; aromatic amino acids; metabolism; shikimate pathway
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Funding
- Israeli Ministry of Industry, Trade and Labor
- Israeli Bio-TOV Consortium
- HAZERA GENETICS Ltd.
- Evogene Ltd.
- FRUTAROM Ltd.
- RAHAN MERISTEM Ltd.
- ZERAIM GEDERA Ltd
- AERI Alternative Sustainable Energy Research Initiative of the Weizmann Institute of Science
- European Research Council (ERC)
- CBE
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The shikimate pathway of plants mediates the conversion of primary carbon metabolites via chorismate into the three aromatic amino acids and to numerous secondary metabolites derived from them. However, the regulation of the shikimate pathway is still far from being understood. We hypothesized that 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (DAHPS) is a key enzyme regulating flux through the shikimate pathway. To test this hypothesis, we expressed a mutant bacterial AroG gene encoding a feedback-insensitive DAHPS in transgenic Arabidopsis plants. The plants were subjected to detailed analysis of primary metabolism, using GC-MS, as well as secondary metabolism, using LC-MS. Our results exposed a major effect of bacterial AroG expression on the levels of shikimate intermediate metabolites, phenylalanine, tryptophan and broad classes of secondary metabolite, such as phenylpropanoids, glucosinolates, auxin and other hormone conjugates. We propose that DAHPS is a key regulatory enzyme of the shikimate pathway. Moreover, our results shed light on additional potential metabolic bottlenecks bridging plant primary and secondary metabolism.
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