Journal
NEW PHYTOLOGIST
Volume 191, Issue 4, Pages 984-995Publisher
WILEY-BLACKWELL
DOI: 10.1111/j.1469-8137.2011.03759.x
Keywords
Arabidopsis; COLD-ON REGULATED (COR) genes; drought; freezing tolerance; germination; osmotic stress; rice (Oryza sativa); SENSITIVE TO FREEZING-6 (SFR6)
Categories
Funding
- Project Sri Lanka
- BBSRC [BB/D009162/1, BB/F01984X/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/F01984X/1, BB/D009162/1] Funding Source: researchfish
Ask authors/readers for more resources
The Arabidopsis protein SENSITIVE TO FREEZING-6 (AtSFR6) is required for cold-and drought-inducible expression of COLD-ON REGULATED (COR) genes and, as a consequence, AtSFR6 is essential for osmotic stress and freezing tolerance in Arabidopsis. Therefore, orthologues of AtSFR6 in crop species represent important candidate targets for future manipulation of stress tolerance. We identified and cloned a homologue of AtSFR6 from rice (Oryza sativa), OsSFR6, and confirmed its orthology in Arabidopsis. OsSFR6 was identified by homology searches, and a full-length coding region isolated using reverse transcription polymerase chain reaction (RT-PCR) from Oryza sativa cDNA. To test for orthology, OsSFR6 was expressed in an Arabidopsis sfr6 loss-of-function mutant background, and restoration of wild-type phenotypes was assessed. Searching the rice genome revealed a single homologue of AtSFR6. Cloning and sequencing the OsSFR6 coding region showed OsSFR6 to have 61.7% identity and 71.1% similarity to AtSFR6 at the predicted protein sequence level. Expression of OsSFR6 in the atsfr6 mutant background restored the wild-type visible phenotype, as well as restoring wild-type levels of COR gene expression and tolerance of osmotic and freezing stresses. OsSFR6 is an orthologue of AtSFR6, and thus a target for future manipulation to improve tolerance to osmotic and other abiotic stresses.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available