Journal
NEW PHYTOLOGIST
Volume 187, Issue 2, Pages 301-312Publisher
WILEY
DOI: 10.1111/j.1469-8137.2010.03275.x
Keywords
Arabidopsis; cell death; low temperature; LSD1; reactive oxygen species (ROS)
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Funding
- National Natural Science Foundation of China [30670181, 90817007]
- National Key Basic Research Program of China [2009CB 119100]
- Ministry of Science and Technology of China [2007AA021402]
- Research Fund for the Doctoral Program of Higher Education of China [20070019002]
- Program for New Century Excellent Talents in University [NCET-05-0124]
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P>In higher plants, the crosstalk between cold stress responses and reactive oxygen species (ROS) signaling is not well understood. Two chilling-sensitive mutants, chs4-1 and chs4-3, were characterized genetically and molecularly. The CHS4 gene, identified by map-based cloning, was found to be identical to LESION SIMULATING DISEASE RESISTANCE 1 (LSD1). We therefore renamed these two alleles lsd1-3 and lsd1-4, respectively. These two mutants exhibited an extensive cell death phenotype under cold stress conditions. Consistently, lsd1-3 plants exposed to cold showed up-regulation of the PR1 and PR2 genes, and increased accumulation of salicylic acid. These results indicate that low temperature is another trigger of cell death in lsd1 mutants. Furthermore, lsd1-3 plants accumulated higher concentrations of H2O2 and total glutathione under cold conditions than wild-type plants. Genetic analysis revealed that PAD4 and EDS1, two key signaling regulators mediating resistance responses, are required for the chilling-sensitive phenotype of lsd1-3. These findings reveal a role of LSD1 in regulating cell death trigged by cold stress and a link between cold stress responses and ROS-associated signaling.
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