Journal
NEW PHYTOLOGIST
Volume 183, Issue 1, Pages 212-223Publisher
WILEY
DOI: 10.1111/j.1469-8137.2009.02835.x
Keywords
arbuscular mycorrhizal fungi (AMF); DNA barcoding; ITS region; LSU rRNA gene; molecular community analyses; rDNA; species level resolution; specific primers
Categories
Funding
- Marie Curie Early Stage Research Training Fellowship of the European Community's Sixth framework Programme [MEST-CT-2005-021016]
Ask authors/readers for more resources
At present, molecular ecological studies of arbuscular mycorrhizal fungi (AMF) are only possible above species level when targeting entire communities. To improve molecular species characterization and to allow species level community analyses in the field, a set of newly designed AMF specific PCR primers was successfully tested. Nuclear rDNA fragments from diverse phylogenetic AMF lineages were sequenced and analysed to design four primer mixtures, each targeting one binding site in the small subunit (SSU) or large subunit (LSU) rDNA. To allow species resolution, they span a fragment covering the partial SSU, whole internal transcribed spacer (ITS) rDNA region and partial LSU. The new primers are suitable for specifically amplifying AMF rDNA from material that may be contaminated by other organisms (e.g., samples from pot cultures or the field), characterizing the diversity of AMF species from field samples, and amplifying a SSU-ITS-LSU fragment that allows phylogenetic analyses with species level resolution. The PCR primers can be used to monitor entire AMF field communities, based on a single rDNA marker region. Their application will improve the base for deep sequencing approaches; moreover, they can be efficiently used as DNA barcoding primers. New Phytologist (2009) 183: 212-223doi: 10.1111/j.1469-8137.2009.02835.x.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available