Journal
NEW PHYTOLOGIST
Volume 185, Issue 3, Pages 676-686Publisher
WILEY
DOI: 10.1111/j.1469-8137.2009.03115.x
Keywords
Chlamydomonas reinhardtii; light-harvesting complex; photosystem; sulfolipid; sulfur-starvation; thylakoid membranes
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Funding
- Ministry of Education, Culture, Sports, Science, and Technology of Japan (MEXT)
- Promotion and Mutual Aid Corporation for Private Schools of Japan
- Japan Science Society
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Regulation of synthesis and degradation of sulfoquinovosyl diacylglycerol (SQDG), one of the membrane lipids that construct thylakoids, under sulfur (S)-starved conditions and its physiological significance were explored in a green alga, Chlamydomonas reinhardtii. Here, we used sac1 and sac3 mutants defective in response to ambient S-status to characterize the system of known induction of SQDG degradation by S starvation that ensures a major S source for protein synthesis. The SQDG synthesis system was monitored in the wild type during S starvation. An SQDG-deficient mutant, hf-2, was utilized to discover functions where SQDG metabolism participates during S starvation. The induction of SQDG degradation was largely repressed in both sac1 and sac3 mutants. The SQDG synthesis capacity was increased by 40% after S starvation, with a sixfold elevation in the mRNA level of the SQD1 gene for SQDG synthesis. Compared with the wild type, hf-2 had decreased protein accumulation, photosystem (PS) I stability and growth rate. A role of SQDG as an S storage lipid is fulfilled under the control of both SAC1 and SAC3 genes, and it is essential for proper protein synthesis in acclimatization of cells to S starvation. The enhancement in SQDG synthesis may reflect the importance of SQDG as the membrane lipid that stabilizes the PSI complex.
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