Journal
NEW BIOTECHNOLOGY
Volume 29, Issue 3, Pages 372-378Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.nbt.2011.03.008
Keywords
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Funding
- National Natural Science Foundation of China [31071559]
- Guangdong Province Cooperation Project of Industry, Education and Academy [2008A010700006]
- Science and Technology Project of Guangdong Province [2009B080701085]
- State Key Laboratory of Pulp and Paper Engineering, SCUT [200823]
- SCUT [2009zm0199, 2009zz0026, 2009zz0018]
- Major State Basic Research Development Program '973' [2010CB732201]
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Oleaginous yeast Trichosporon fermentans was proved to be able to use sulphuric acid-treated sugar cane bagasse hydrolysate as substrate to grow and accumulate lipid. Activated charcoal was shown as effective as the more expensive resin Amber lite XAD-4 for removing the inhibitors from the hydrolysate. To further improve the lipid production, response surface methodology (RSM) was used and a 3-level 4-factor Box-Behnken design was adopted to evaluate the effects of C/N ratio, inoculum concentration, initial pH and fermentation time on the cell growth and lipid accumulation of T. fermentans. Under the optimum conditions (C/N ratio 165, inoculum concentration 11%, initial pH 7.6 and fermentation time 9 days), a lipid concentration of 15.8 g/L, which is quite close to the predicted value of 15.6 g/L, could be achieved after cultivation of T. fermentans at 25 degrees C on the pretreated bagasse hydrolysate and the corresponding lipid coefficient (lipid yield per mass of sugar, %) was 14.2. These represent a 32.8% improvement in the lipid concentration and a 21.4% increase in the lipid coefficient compared with the original values before optimization (11.9 g/L and 11.7). This work further demonstrates that T. fermentans is a promising strain for lipid production and thus biodiesel preparation from abundant and inexpensive lignocellulosic materials.
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