Journal
NEW BIOTECHNOLOGY
Volume 28, Issue 2, Pages 165-172Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.nbt.2010.10.009
Keywords
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Funding
- Department of Food Science and Engineering, Faculty of Agricultural Engineering and Technology, University of Tehran
- Agricultural Biotechnology Research Institute of Iran (ABRII)
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Brewer's spent grain (BSG) was used as a solid substrate for the production of alpha-amylase by Bacillus sp. KR-8104 in a submerged fermentation system. The production of alpha-amylase was maximized through statistical optimization of the BSG concentration and incubation time using the Doehlert experimental design. The highest tested amount of BSG (5%, w/v) in the optimization process resulted in a 5.1-fold enhancement of the response. Subsequently, we studied the role of the water-soluble and -insoluble fractions of BSG in the production of alpha-amylase. The results revealed that whole BSG had a greater effect on the production of alpha-amylase than each fraction had separately. Finally, when we examined the potential of BSG to replace the constituents of a medium formula, we observed that simultaneously adding BSG, omitting dextrin, and reducing the other ingredients concentration in the culture medium improved the production of alpha-amylase and made the production process more economical.
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