Journal
NEW BIOTECHNOLOGY
Volume 27, Issue 3, Pages 212-221Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.nbt.2010.03.003
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Funding
- Research Council of Norway
- Yale University
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The ability to interfere with gene expression is of crucial importance to unravel the function of genes and is also a promising therapeutic strategy. Here we discuss methodologies for inhibition of target RNAs based on the cleavage activity of the essential enzyme, Ribonuclease P (RNase P). RNase P-mediated cleavage of target RNAs can be directed by external guide sequences (EGSs) or by the use of the catalytic M1 RNA from E. coli linked to a guide sequence (M1GSs). These are not only basic tools for functional genetic studies in prokaryotic and eukaryotic cells but also promising antibacterial, anticancer and antiviral agents.
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