Journal
STEM CELLS
Volume 33, Issue 11, Pages 3197-3204Publisher
WILEY
DOI: 10.1002/stem.2123
Keywords
Cell surface markers; Stem cell-microenvironment interactions; Cell cycle; Cell biology; Adult stem cells
Categories
Funding
- NIH K99-R00 [R00-AR063127]
- Milton Fund Award
- Smith Family New Investigator Award
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Lineage tracing is a method that delineates all progeny produced by a single cell or a group of cells. The possibility of performing lineage tracing initiated the field of Developmental Biology and continues to revolutionize Stem Cell Biology. Here, I introduce the principles behind a successful lineage-tracing experiment. In addition, I summarize and compare different methods for conducting lineage tracing and provide examples of how these strategies can be implemented to answer fundamental questions in development and regeneration. The advantages and limitations of each method are also discussed.
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