Journal
NEW BIOTECHNOLOGY
Volume 25, Issue 2-3, Pages 126-132Publisher
ELSEVIER
DOI: 10.1016/j.nbt.2008.08.004
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Funding
- BBSRC
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Protein production is one of the key steps in biotechnology and functional proteomics. Expression of proteins in heterologous hosts (such as in E. coli) is generally lengthy and costly. Cell-free protein synthesis is thus emerging as an attractive alternative. In addition to the simplicity and speed for protein production, cell-free expression allows generation of functional proteins that are difficult to produce by in vivo systems. Recent exploitation of cell-free systems enables novel development of technologies for rapid discovery of proteins with desirable properties from very large libraries. This article reviews the recent development in cell-free systems and their application in the large scale protein analysis.
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