Journal
STEM CELL REVIEWS AND REPORTS
Volume 11, Issue 2, Pages 242-253Publisher
SPRINGER
DOI: 10.1007/s12015-014-9581-5
Keywords
Pluripotent stem cell; Mesenchymal stem/stromal cell; Cartilage tissue engineering; MRI (magnetic resonance imaging); Osteoarthritis
Funding
- National Institute of Arthritis and Musculoskeletal and Skin Diseases [NIH R21AR059861-01, 2R01AR054458-05, NIH R01 HL113006, NIH U01 HL099776]
Ask authors/readers for more resources
Human induced pluripotent stem cells (hiPSCs) have demonstrated great potential for hyaline cartilage regeneration. However, current approaches for chondrogenic differentiation of hiPSCs are complicated and inefficient primarily due to intermediate embryoid body formation, which is required to generate endodermal, ectodermal, and mesodermal cell lineages. We report a new, straightforward and highly efficient approach for chondrogenic differentiation of hiPSCs, which avoids embryoid body formation. We differentiated hiPSCs directly into mesenchymal stem /stromal cells (MSC) and chondrocytes. hiPSC-MSC-derived chondrocytes showed significantly increased Col2A1, GAG, and SOX9 gene expression compared to hiPSC-MSCs. Following transplantation of hiPSC-MSC and hiPSC-MSC-derived chondrocytes into osteochondral defects of arthritic joints of athymic rats, magnetic resonance imaging studies showed gradual engraftment, and histological correlations demonstrated hyaline cartilage matrix production. Results present an efficient and clinically translatable approach for cartilage tissue regeneration via patient-derived hiPSCs, which could improve cartilage regeneration outcomes in arthritic joints.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available