Journal
NEUROTOXICOLOGY
Volume 45, Issue -, Pages 38-47Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.neuro.2014.09.003
Keywords
Glufosinate; Neurotoxicity; N-Methyl-D-aspartate receptor; N-Acetylglufosinate
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Funding
- STAR Fellowship Assistance - U.S. Environmental Protection Agency (EPA) [FP917139]
- UC Berkeley Sponsored Project for Undergraduate Research (SPUR) Grant
- EPA [FP917139, 672975] Funding Source: Federal RePORTER
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Glufosinate (GLF) at high levels in mammals causes convulsions and amnesia through a mechanism that is not completely understood. The structural similarity of GLF to glutamate (GLU) implicates the glutamatergic system as a target for GLF neurotoxicity. The current work examined in vitro GLF interaction with N-methyl-D-aspartate subtype GLU receptors (NMDARs) and GLT-1 transporters via [H-3]CGP 39653 binding experiments and [H-3]GLU uptake assays, respectively. GLF effects on neuronal network activity were assessed using microelectrode array (MEA) recordings in primary cultures of cortical neurons. GLF and its primary metabolite N-acetylglufosinate (NAcGLF) bind to the NMDAR; the IC50 value for GLF was 668 mu M and for NAcGLF was about 100 mu M. Concentrations of GLF greater than 1000 mu M were needed to decrease,GLU uptake through GLT-1. In MEA recordings from networks of rat primary cortical neurons, the concentration-responses for NMDA, GLF and NAcGLF on network mean firing rates (MFR) were biphasic, increasing at lower concentrations and decreasing below control levels at higher concentrations. Increases in MFR occurred between 3-10 mu M NMDA (290% control, maximum), 100-300 mu M NAcGLF (190% control, maximum) and 10-1000 mu M GLF (340% control, maximum). The NMDAR antagonist MK801 attenuated both NMDA and GLF increases in MFR. The GLF concentration required to alter GLU transport through GLT-1 is not likely to be attained in vivo, and therefore not relevant to the neurotoxic mode of action. However, toxicokinetic data from reports of intentional human poisonings indicate that GLF concentrations in the CNS after acute exposure could reach levels high enough to lead to effects mediated via NMDARs. Furthermore, the newly characterized action of NAcGLF at the NMDAR suggests that both the parent compound and metabolite could contribute to neurotoxicity via this pathway. Published by Elsevier Inc.
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