Journal
NEUROSURGERY
Volume 70, Issue 1, Pages 188-197Publisher
OXFORD UNIV PRESS INC
DOI: 10.1227/NEU.0b013e3182262c5b
Keywords
Anticancer chemotherapy; Brain tumors; Cell culture; Confocal microscopy
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Funding
- [DPT 2003K120470-37]
- [DPT 2003K-121470-25]
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BACKGROUND: Anaplastic pleomorphic xanthoastrocytoma is an aggressively growing, malignant, and eventually fatal tumor of the central nervous system. Testing chemotherapeutic drug sensitivity under in vitro conditions would be a useful strategy to determine sensitive or resistant drugs for fatal brain cancers. OBJECTIVE: To establish primary cell cultures of excised tumor tissue from pleomorphic xanthoastrocytoma-bearing patients and to test their sensitivity against various anticancer chemotherapy drugs. METHODS: Prepared suspensions of the excised tumor tissue from a patient who had a recurrent grade 3 pleomorphic xanthoastrocytoma was cultured in culture dishes until cells began to grow. Immunofluorescent and immunohistochemical visualizations were performed using confocal and light microscopy. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay in comparison with 3 H-thymidine incorporation assay was used to test cellular toxicity of several anticancer drugs. RESULTS: We established vigorously growing primary cells of the tumor. Drug sensitivity testing was conducted successfully. CONCLUSION: Primary cell cultures of surgically removed tumor tissues may be useful in studies of cancer biology and chemotherapeutic drug sensitivity for recurrent malignant brain tumors, particularly for anaplastic pleomorphic xanthoastrocytoma.
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