Cellular distribution of the nicotinic acetylcholine receptor α7 subunit in rat hippocampus

The hippocampus is a region of the mammalian brain that has been extensively studied due to its role in many forms of memory. To better understand hippocampal function, significant attention has focused upon the cellular distribution of ligand-gated ion channels. Despite strong cholinergic innervation from the basal forebrain and a dense expression of nicotinic acetylchoine receptors (nAChRs), the cellular distribution of subunits forming these receptors has received little attention. We used organotypic hippocampal slice Cultures (OHSCs) to study native alpha 7 subunits, which, unlike other nAChR subunits, form a homomeric receptor Cell-surface biotinylation, cross-linking of surface proteins, and sub-cellular fractionation all revealed a very limited presence of the subunit at the plasma membrane. In contrast, subunits of other receptors displayed significant surface expression Notably, subunits in adult hippocampal tissue were distributed in a fashion similar to that observed in OHSCs. To monitor alpha 7 subunits contained in functional nAChRs, a colourimetric assay using alpha-bungarotoxin (a specific alpha 7 nAChR antagonist) was developed, and revealed a majority of binding at the cell surface. To change alpha 7 subunit distribution, OHSCs were treated with compounds known to affect other ionotropic receptors-insulin, genistein, and elevated external K+; however. neither subunit surface expression nor antagonist binding was affected. Our data reveal that hippocampal neurons possess a large internal population of alpha 7 subunits under basal conditions, which persists during stimuli affecting tyrosine phosphorylation or neuronal activity. The nature of the internal pool of alpha 7 subunits remains to be determined, but should have important implications for hippocampal activity. (C) 2009 Elsevier Ireland Ltd and the Japan Neuroscience Society All rights reserved.