Journal
NEUROSCIENCE RESEARCH
Volume 64, Issue 3, Pages 290-296Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neures.2009.03.014
Keywords
Growth cone; Guidance; Scanning ion conductance microscopy; Leech
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Funding
- Italian Ministero dell'istruzione
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Scanning ion conductance microscopy has been applied to neuronal growth cones of the leech either to image or to stimulate them. Growth cone advance was recorded in non-contact mode using a 2% ion current decrease criterion for pipette-membrane distance control. We demonstrate effective growth cone remodelling using a 5% criterion (near-scanning). Recurrent line near-scanning aligned growth cone processes along the scan line. The new membrane protrusions, marked by Dil, started a few minutes after scanning onset and progressively grew in thickness. Using scanning patterns suitable for connecting distinct growth cones, new links were consistently developed. Although the underlying mechanism is still a matter for investigation, a mechanical perturbation produced by the moving probe appeared to induce the process formation. Thanks to its deterministic and interactive features, this novel approach to guiding growth cones is a promising way to develop networks of identified neurons as well as link them with artificial structures. (c) 2009 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.
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