Journal
NEUROSCIENCE LETTERS
Volume 548, Issue -, Pages 61-66Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neulet.2013.05.047
Keywords
Calcium-sensitive dye; DYT1 dystonia; Fluorescence imaging; Glutamate receptor; Neuron
Categories
Funding
- American Heart Association
- Dystonia Medical Research Foundation
- Edward Mallinckrodt, Jr. Foundation
- National Science Foundation
- Whitehall Foundation
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Increased activities of cytoplasmic calcium and the excitatory neurotransmitter glutamate have been independently implicated in dystonia pathophysiology. However, cellular-level evidence linking these two features is not available. Here we show that glutamate-dependent changes in neuronal calcium dynamics occur in a knock-in mouse model of DYT1 dystonia, the most common hereditary form of this disorder. Fluorescence-based analysis of the dynamics of cytoplasmic calcium concentration ([Ca2+](c)) in cultured hippocampal neurons shows that electrical stimulation depolarizes the neurons and increases the dendritic [Ca2+](c), which then decays slowly to the pre-stimulus level. Whereas the peak amplitude of [Ca2+](c) was not affected, the decay period was prolonged in neurons of heterozygous mice whose genotype reflects the human condition. We found that this effect was blocked by the antagonists of ionotropic glutamate receptors, and confirmed that glutamate receptors are present in these neurons. As the [Ca2+](c) is readout and regulator of neuronal excitability, its abnormality represents an important cellular phenotype of dystonia (C) 2013 Elsevier Ireland Ltd. All rights reserved.
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