Journal
NEUROSCIENCE LETTERS
Volume 465, Issue 1, Pages 1-5Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neulet.2009.08.074
Keywords
DJ-1; Oxidative stress; Parkinson's disease; Biomarker; Erythrocytes; Enzyme-linked immunosorbent assay
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Funding
- Ikeda Municipal Hospital
- Toneyama National Hospital
- Japan Society for the Promotion of Science [18790081, 19300256]
- New Energy and Industrial Technology Development Organization (NEDO)
- Grants-in-Aid for Scientific Research [18790081, 19300256] Funding Source: KAKEN
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DJ-1 was initially identified as a novel oncogene and has recently been found to be a causative gene for a familial form of Parkinson's disease (PD), viz, PARK7. Cysteine residue at position 106 (Cys-106) in DJ-1 was found to be oxidized preferentially under oxidative stress. In the present study, we developed specific antibodies against Cys-106-oxidized DJ-1 using baculovirus particles displaying the surface glycoprotein gp64-fusion protein as the immunizing agent. Western blot analysis combined with two-dimensional gel electrophoresis revealed that these antibodies specifically recognized oxidized DJ-1. Furthermore, we developed a competitive enzyme-linked immunosorbent assay (ELISA) for detecting oxidized DJ-1 and measured blood levels of oxidized DJ-1 in PD patients (n=15). It was observed that the levels of oxidized DJ-1 in erythrocytes of unmedicated PD patients were markedly higher without overlap than those of medicated PD patients and healthy subjects. No significant difference was observed in DJ-1 levels between mediated and unmediated PD patient. These results suggest the oxidative modification of DJ-1 in PD patients and the potential application of the antibody for diagnosis of PD at early-stage. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
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