Journal
NEUROSCIENCE LETTERS
Volume 465, Issue 2, Pages 128-132Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neulet.2009.08.069
Keywords
Granulocyte-colony stimulating factor; Lipopolysaccharide; Neuron; Neuroprotection
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Funding
- National Natural Science Foundation of China [30572164]
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Granulocyte-colony stimulating factor (G-CSF) has recently been noted for neuroprotective function. Evidence has been given to indicate that G-CSF is naturally expressed in neurons and directly activates anti-apoptosis pathways. Finding out the agents inducing G-CSF production is of value for understanding the neuroprotection network in central nervous system It is known that lipopolysaccharide (LPS) can induce macrophages to produce G-CSF. Here we demonstrate that hippocampal neurons exhibited the expression of toll-like receptor-4. and prove that low-dose LPS treatment increased the expression and production of G-CSF mRNA and protein in cultured neurons. We further indicate that the neutralization of G-CSF with corresponding anti-G-CSF antibodies abolished the neuroprotective effect of LPS pretreatment in N-methyl-n-aspartic acid-induced neuronal injury by MTT/CCK-8 assays and LDH release. Thus our results reveal that G-CSF may be involved in LPS-mediated neuroprotection in vivo. (c) 2009 Elsevier Ireland Ltd. All rights reserved
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