Journal
NEUROSCIENCE LETTERS
Volume 433, Issue 3, Pages 231-234Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neulet.2008.01.012
Keywords
apoptosis; cell death; avulsion; dorsal horn; dorsal root ganglion
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Flow cytometry and terminal deoxynucleotidyl transferase-mediated biotinylated uridine triphosphate nick end-labelling (TUNEL) immuno-histochemistry have been used to assess cell death in the dorsal root ganglia (DRG) or spinal cord 1, 2 or 14 days after multiple lumbar dorsal root rhizotomy or dorsal root avulsion injury in adult rats. Neither injury induced significant cell death in the DRG compared to sham-operated or naive animals at any time point. In the spinal cord, a significant increase in death was seen at 1-2 days, but not 14 days, post injury by both methods. TUNEL staining revealed that more apoptotic cells were present in the dorsal columns and dorsal horn of avulsion animals compared to rhizotomised animals. This suggests that avulsion injury, which can often partially damage the spinal cord, has more severe effects on cell survival than rhizotomy, a surgical lesion which does not affect the spinal cord. The location of TUNEL positive cells suggests that both neuronal and non-neuronal. cells are dying. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
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