Journal
NEUROSCIENCE
Volume 272, Issue -, Pages 29-33Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuroscience.2014.04.050
Keywords
main olfactory epithelium; olfactory receptor neurons; total neuron number; stereology; mouse
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Funding
- Ministry of Education, Science, Sports and Culture of Japan [23791886, 25462661]
- Grants-in-Aid for Scientific Research [23791886, 25462661] Funding Source: KAKEN
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The total number of olfactory receptor neurons (ORNs) in the mouse main olfactory epithelium (MOE) was estimated using stereological sampling. Noses and skulls of male and female 8-week-old C57BL/6J mice were de-calcified, embedded in paraffin, cut into 10-mu m-thick sections serially at 100-mu m intervals, and processed for immunohistochemistry for the olfactory marker protein (OMP), a specific marker for ORNs. The number of OMP (+) receptor neurons was measured using an optical fractionator with the Stereo-Investigator software. The mean values of the total number of OMP (+) receptor neurons in the unilateral MOE were 5,140,000 +/- 380,000 in males and 5,210,000 +/- 380,000 in females, with no significant differences between the sexes. We concluded that the total number of ORNs in the unilateral MOE is approximately 5 x 106 in mice. (C) 2014 IBRO. Published by Elsevier Ltd. All rights reserved.
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