INHIBITION OF ENDOPLASMIC RETICULUM CA2+ ATPASE IN PREBOTZINGER COMPLEX OF NEONATAL RAT DOES NOT AFFECT RESPIRATORY RHYTHM GENERATION

PreBotzinger complex (preBotC) neurons in the brainstem underlie respiratory rhythm generation in vitro. As a result of network interactions, preBotC neurons burst synchronously to produce rhythmic premotor inspiratory activity. Each inspiratory neuron has a characteristic 10-20 mV, 0.3-0.8 s synchronous depolarization known as the inspiratory drive potential or inspiratory envelope, topped by action potentials (APs). Mechanisms involving Ca2+ fluxes have been proposed to underlie the initiation of the inspiratory drive potential. An important source of intracellular Ca2+ is the endoplasmic reticulum (ER) in which active Ca2+ sequestration is mediated by a class of transporters termed sarco/endoplasmic reticulum Ca2+ ATPases (SERCAs). We aim to test the hypothesis that disruption of Ca2+ sequestration into the ER affects respiratory rhythm generation. We examined the effect of inhibiting SERCA on respiratory rhythm generation in an in vitro slice preparation. Bath application of the potent SERCA inhibitors thapsigargin or cyclopiazonic acid (CPA) for up to 90 min did not significantly affect the period or amplitude of respiratory-related motor output or integral and duration of inspiratory drive in preBotC neurons. We promoted the depletion of intracellular Ca2+ stores by a transient bath application of 30 mM K+ (high K+) in the continuous presence of thapsigargin or CPA. After washing out the high K+, respiratory rhythm period and amplitude returned to baseline values. These results show that after inhibition of SERCA and depletion of intracellular Ca2+ stores, respiratory rhythm remains substantially the same, suggesting that this source of Ca2+ does not significantly contribute to rhythm generation in the preBotC in vitro. (C) 2012 IBRO. Published by Elsevier Ltd. All rights reserved.