4.5 Article

HETEROGENOUS GABAB RECEPTOR-MEDIATED PATHWAYS ARE INVOLVED IN THE LOCAL GABAERGIC SYSTEM OF THE RAT TRIGEMINAL GANGLION: POSSIBLE INVOLVEMENT OF KCTD PROTEINS

Journal

NEUROSCIENCE
Volume 218, Issue -, Pages 344-358

Publisher

PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuroscience.2012.05.038

Keywords

GABA; sensory ganglion; satellite cell; neuron-glia interaction; K+ channel tetramerization domain-containing protein

Categories

Funding

  1. Osaka Medical Research Foundation for Incurable Disease
  2. Ministry of Education, Sports, Culture, Science and Technology of Japan (High-Tech Research Program of Osaka Medical College)
  3. Japan Society for the Promotion of Science [19591824, 21591990]
  4. Keio Gijuku Academic Development Funds
  5. Fukuzawa Memorial Fund for the Advancement of Education and Research
  6. Grants-in-Aid for Scientific Research [19591824, 21591990, 24592319] Funding Source: KAKEN

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It is well known that Gamma-aminobutyric acid (GABA) plays an important role in signal transduction in the central nervous system. However, the function of GABA in the peripheral nervous system, including sensory ganglions, is still unclear. In this study we have characterized the expression, cellular distribution, and function of GABA(B) receptor subunits, and the recently discovered GABA(B) auxiliary subunits, K+ channel tetramerization domain-containing (KCTD) proteins, in rat trigeminal ganglion (TG) neuronal cells, which are devoid of synapses. We found heterogeneous expression of both GABA(B1) and GABA(B2) subunits, and a near-plasma membrane localization of KCTD12. In addition, we found that GABA(B2) subunits correlated with KCTD16. Whole-cell current-clamp recordings showed that responses to the GABA(B) receptor agonist, baclofen, were variable and both increases and decreases in excitability were observed. This correlated with observed differences in voltage-dependent K+ current responses to baclofen in voltage-clamped TG neuronal cells. The functional diversity of the GABA(B)ergic regulation on the excitability of the TG neuronal cell bodies could be due to the heterogenous expression of KCTD proteins, and subsequent regulation of plasma membrane K+ channels. Taken together with our previous demonstration of a local GABA(A) receptor-mediated system in rat TG, we provide an updated GABAergic model in the rat TG that incorporates both GABA(A)- and GABA(B)-receptor systems. (C) 2012 IBRO. Published by Elsevier Ltd. All rights reserved.

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