Journal
NEUROREPORT
Volume 23, Issue 3, Pages 184-188Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/WNR.0b013e32834faab0
Keywords
acetylcholine; basal ganglia; GABAergic inhibitory postsynaptic current; opioid; patch/striosome; striatum
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Funding
- Ministry of Education, Culture, Sports, Science, and Technology of Japan
- Japan Society for the Promotion of Science
- Smoking Research Foundation of Japan
- Grants-in-Aid for Scientific Research [21500362, 21200015] Funding Source: KAKEN
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We have previously reported that earlier blockade of protein kinase C (PKC) augments the suppressive effect of l-opioid receptors (MORs) on the GABAergic inhibitory postsynaptic current (IPSC) in the MOR-rich striosomes of the striatum. Interestingly, striatal medium-spiny neurons have muscarinic acetylcholine receptor subtypes M-1 and M-4, among which M-1 activates the phosphoinositide signaling pathway yielding PKC. In this study, we examined whether acetylcholine regulates the effects of MOR on presynaptic IPSC by binding to the M-1 receptor, and found that IPSC suppression by the MOR agonist, [D-Ala(2)-N-Me-Phe(4), Gly(5)-ol]-enkephalin, was significantly augmented and prolonged by the PKC inhibitor chelerythrine and attenuated by the PKC activator, phorbol 12, 13-dibutyrate. This modulatory action by chelerythrine was mimicked by the muscarinic antagonist atropine and the M-1-specific antagonist pirenzepine, whereas M-2-M-4 antagonists had no discernible effect. These results suggest that PKC activity modulates the effect of MOR by muscarinic receptors in the striosomes. NeuroReport 23: 184-188 (C) 2012 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.
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